22.03.2020 03
KhoomAbstract
Txhawm rau kawm txog kev ua haujlwm hauv cheeb tsam thiab kev taw qhia ntawm cov proteins hauv cov qauv subcellular, peng Xi thiab nws cov npoj yaig tau tsim lub teeb pom kev zoo polarized (pSIM).Txoj kev tshawb no yog nyob rau hauv phau ntawv Journal Nature Communications.
Qhov kev ua haujlwm no "ua kom muaj zog" rau SIM uas twb muaj lawm los ntawm kev tshawb fawb tob txog cov yam ntxwv ntawm SIM technology thiab cov cuab yeej ua lag luam, thiab tshawb nrhiav cov yam ntxwv ntawm lub cev muaj zog ntawm SIM uas twb muaj lawm uas txawm tias nws cov neeg tsim khoom tsis tau pom, yog li ntawd cov txheej txheem uas twb muaj lawm tuaj yeem paub txog cov yam ntxwv ntawm tus qauv SIM uas twb muaj lawm. muaj nuj nqi ntawm polarization SIM tsis muaj kev hloov kho.Qhov no ua rau ntau lub chaw soj nstuam hauv lub neej nrog SIM systems los txheeb xyuas cov polarization SIM ncaj qha, uas yuav ua rau kev tshawb fawb ntawm polarization super-resolution imaging.
Fig.1Imaging lub orientation ntawm luv actin filaments.Dynamic imaging ntawm myosin-tsav txav ntawm phalloidin-labeled actin filament.Lub thawv dawb muaj cov trajectory ntawm luv luv actin filament.b Magnified saib ntawm lub thawv daj hauv cheeb tsam a.Dipole orientations ntawm actin filaments hloov thaum lawv txav mus.c Time-lapse orientation thiab txoj hauj lwm ntawm fragmented actin hauv b.d 2D-pSIM imaging ntawm actin filaments nyob rau hauv hippocampal neurons, uas qhia meej qhov txawv ntawm qhov nruam ntev actin filaments nyob rau hauv lub dendrite thiab cheeb tsam ntawm discrete actin nplhaib qauv nyob rau hauv lub axon.e Lwm qhov kev piav qhia ntawm lub nplhaib actin hauv lub axon.f, g Magnified views ntawm thaj tsam boxed hauv d, uas piv cov txiaj ntsig ntawm PM thiab pSIM imaging.h Qhov kev siv qhov profile ntawm kab qhia hauv e, nws qhov Fourier hloov pauv (i) qhia txog 184 nm ncua sij hawm, raws li cov txiaj ntsig tau tshaj tawm yav dhau los.j, k Cov qauv ntawm lub nplhaib actin yog qhov tseem ceeb rau cov txheej txheem ntawm cov txheej txheem ntawm lub cev (MPS) hauv cov neurons.Cov qauv yav dhau los xav tias qhov kawg-rau-kawg lub koom haum rau cov adducin-capped actin filaments.Txawm li cas los xij, pSIM qhia tau hais tias kev taw qhia ntawm luv luv actin filaments yog sib npaug rau axon ncej, txhawb nqa ib sab ntawm lub koom haum rau cov qauv actin nplhaib.Scale bars: a 2 μm thiab d-g 1 μm
Kev tsom xam ntawm kev siv technology
QSIM super-resolution imaging optical system yog ib qho kev pom tsis tshua muaj teeb pom kev zoo.TUCSENDhyana 400BSILub koob yees duab muaj lub ncov quantum efficiency ntawm 95% thiab nyeem cov suab nrov tsawg li 1.2e-, uas tuaj yeem pab lub teeb pom kev qis kom tau txais cov teeb liab-rau-nruj piv cov duab duab.Lub pixel ntawm 6.5 μm yog haum rau 60x lub hom phiaj lub hom phiaj siab.Nws tuaj yeem muab kev ua si tag nrho rau qhov zoo ntawm lub lens daws teeb meem thiab pab lub kaw lus kom tau txais cov duab daws teeb meem siab nrog cov ntsiab lus meej.
Kev siv qhov chaw
Zhanghao K, Chen X. Super-resolution imaging ntawm fluorescent dipoles ntawm polarized structured illumination microscopy.Nat Commun.2019 Kaum Ib Hlis 16; 10(1): 4694.doi: 10.1038/s41467-019-12681-w.PIB: 31619676;PIB: PMC6795901.
